Ku protein
DNA-dependent protein kinase (DNA-PK) is a complex of DNA-PK catalytic subunit (DNA-PKcs) and the DNA end-binding Ku70/Ku80 heterodimer. DNA-PK is required for DNA double strand break repair by the process of nonhomologous end joining. Nonhomologous end joining is a major mechanism for the repair of DNA double strand breaks in mammalian cells.A binding site for the telomere protein TRF2 is located in the N-terminal half of human Ku70 (Song et al., 2000) and, hence, the presence of a truncated Ku protein could potentially contribute to the unique telomere phenotype associated with ku70 mutants (see below). Requirement for the NHEJ pathway varies during plant developmentThis review focuses on DNA-dependent protein kinase (DNA-PK), which is the key regulator of canonical non-homologous end-joining (NHEJ), the predominant mechanism of DNA double-strand break (DSB) repair in mammals. DNA-PK consists of the DNA-binding Ku70/80 heterodimer and the catalytic subunit DNA-PKcs. They assemble at DNA ends, forming the active DNA-PK complex, which ...
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Ara h 3. • Sensitization to Ara h 1, 2 and 3 is usually acquired in childhood. 16. • IgE to Ara h 2 has the best discriminative ability of all diagnostic tests for peanut allergy. It can accurately diagnose peanut allergy in 28% of patients but cannot be used to exclude a peanut allergy in an adult population. 6,12-16.In this study, recombinant Pichia pastoris was constructed for malic acid production from sole methanol through rational redistribution of metabolic flux. Different malic acid accumulation modules were systematically evaluated and optimized in P. pastoris. The recombinant PP-CM301 could produce 8.55 g/L malic acid from glucose, …Coilin is a nuclear protein that plays a role in Cajal body formation. The function of nucleoplasmic coilin is unknown. Here we report that coilin interacts with Ku70 and Ku80, which are major players in the DNA repair process. Ku proteins compete with SMN and SmB′ proteins for coilin interaction sites.Eight sera contained isolated antibodies to Ku proteins: both subunits were recognized in 7 cases, while isolate reactivity to the 70 kDa protein was detected in one case. Five sera contained additional antibody specificities: anti-Ro 60 kDa in 4 cases, and anti-La/SSB, anti-SL, and anti-PM-Scl in one case each.Europe PMC is an archive of life sciences journal literature. Search life-sciences literature (41,454,239 (41,454,239Ku, also known as nuclear Factor IV, is an abundant nuclear DNA-binding protein which requires free DNA ends for the initial interaction with double-stranded DNA (dsDNA) and can bind at multiple sites along dsDNA in an energy-independent manner.Nov 12, 2021 · Collectively, our data show that zinc-finger protein ZNF384 is an adapter of Ku to DNA during DSB repair via cNHEJ. Results. ZNF384 is recruited to DNA damage sites and interacts with NHEJ proteins.We find that a cDNA clone for the smaller subunit (beta) of YPF1 encodes a 72-kDa protein that has extensive homology to the smaller subunit of the heterodimeric Ku protein (24% identity, 51% ...Mar 25, 2019 · a yeast homologue of the 70K subunit of the mammalian Ku protein, which can bind to the ends of double-stranded DNA and mediates double-strand break repair and immunoglobulin V(D)J recombination8 ...The protein expression of Ku 70 and Ku 80 was closely related and decreased consistently with increasing grades and stages, paralleling the expression of the mRNA. Among hazardous environmental bladder cancer risk factors, heavy consumption of coffee was associated with a twofold decreased Ku 70 and Ku 80 mRNA expression, whereas tobacco smoke ...DNA-PK is a trimeric serine-threonine protein kinase consisting of an approx. 470 kDa catalytic polypeptide referred to as DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and a DNA-binding dimer of 70 and 86 kDa polypeptides known as Ku [8], [17].9 Agu 2001 ... The Ku heterodimer, consisting of two subunits, Ku70 and Ku80, is a member of a family of DNA repair proteins that fixes damaged DNA in order to ...Unlike Escherichia coli, Mycobacterium tuberculosis (Mt) expresses a Ku-like protein and an ATP-dependent DNA ligase that can perform non-homologous end-joining (NHEJ). We have expressed the Mt-Ku and Mt-Ligase D in E. coli using an arabinose-inducible promoter and expression vectors that integrate into specific sites in the E. coli chromosome.E. coli strains have been generated that express ...Ku is a heterodimeric protein composed of ~70-and ~80-kDa subunits (Ku70 and Ku80) originally identified as an autoantigen recognized by the sera of patients with autoimmune diseases. Ku has high binding affinity for DNA ends and that is why originally it was known as a DNA end binding protein, but now it is known to also bind the DNA structure ...Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids. Nucleic Acids Res. 1998;26:1551-1559. [PubMed] [DOI]. 8 ...In most cells, Ku (an heterodimer of two tightly associated sub-units called Ku70 and. Ku80), is an abondant protein located in the nucleus, where it plays a ...
Critical to NHEJ is the DNA-dependent interaction of the Ku70/80 heterodimer with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to form the DNA-PK holoenzyme. However, precisely how Ku recruits DNA-PKcs to DSBs ends to enhance its kinase activity has remained enigmatic, with contradictory findings reported in the literature.In this study, we have studied the interaction of Ku with nucleic acids quantitatively by following the change in fluorescence anisotropy of the DNA duplex associated with protein binding. The binding of Ku to nucleic acids occurs at DNA ends and is independent of their structure and sequence (. 10. The Ku protein complex is involved in length regulation of Drosophila telomeres. Genetics 170 , 221-235 (2005). Article CAS PubMed PubMed Central Google ScholarWhen Ku protein was fractionated electrophoretically, transferred to nitrocellulose filter, and probed with 32P-labeled DNA, only the 70,000-dalton subunit exhibited DNA binding. Thus, the Ku protein appears to recognize selectively ends of double-stranded DNA molecules. Possible functions of the Ku autoantigen in eukaryotic cells are discussed.
Therefore, Ku protein neddylation is associated with Ku protein ubiquitination, which may induce apoptotic cell death. Astaxanthin, an oxygen-containing carotenoid (xanthophyll), is present in marine algae, red yeast, fungi, and various plant and animal sources. It is a fat-soluble nutrientThe Ku protein consists of two tightly associated homologous subunits, Ku70 and Ku80, and was originally identified as an autoantigen recognized by the sera of patients with an autoimmunity disease. In eukaryotes, the Ku heterodimer contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by ...The Ku protein-DNA-dependent protein kinase system is one of the major pathways by which cells of higher eukaryotes respond to double-strand DNA breaks. The components of the system are evolutionarily conserved and homologs are known from a number of organisms. The Ku protein component binds directly to DNA ends and may help align them for ...…
Reader Q&A - also see RECOMMENDED ARTICLES & FAQs. Autoantibodies to the DNA-binding Protei. Possible cause: Synthetic trans-(±)-kusunokinin ((±)KU), a potential anticancer substance, w.
Protein-peptide interactions play a fundamental role in many cellular processes, but remain underexplored experimentally and difficult to model computationally. Here, we present PepNN-Struct and ...Synthetic trans-(±)-kusunokinin ((±)KU), a potential anticancer substance, was revealed to have an inhibitory effect on breast cancer.According to the computational modeling prediction, AKR1B1, an oxidative stress and cancer migration protein, could be a target protein of trans-(−)-kusunokinin.In this study, we determined the binding of (±)KU and AKR1B1 on triple-negative breast and non ...The Ku protein has a critical function in the repair of double-strand DNA breaks induced for example by ionizing radiation or during VDJ recombination. Ku serves as the DNA-binding subunit of the DNA-dependent kinase and is a heterodimeric protein composed of 80- and 70-kDa subunits. We used the two-hybrid system to analyze the interaction ...
Here, small angle X-ray scattering (SAXS) and mutational analyses show APLF is largely an intrinsically disordered protein that binds Ku, Ku/DNA-PKcsmore » X4L4 assembles with Ku heterodimers linked to DNA-PKcs via flexible Ku80 C-terminal regions (Ku80CTR) in a complex stabilized through APLF interactions with Ku, DNA-PK, and X4L4.The Mechanism of V(D)J Recombination. JOANN SEKIGUCHI, ... MARJORIE OETTINGER, in Molecular Biology of B Cells, 2004. Ku70 and Ku80. Ku70 and Ku80 form a heterodimer, Ku, which possesses DNA end-binding activity (Mimori and Hardin, 1986).Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was …
Aravind L, Koonin EV . 2001 Prokaryotic homologs of the eu Ku is a heterodimeric protein with double-stranded DNA end-binding activity that operates in the process of nonhomologous end joining. Ku is thought to target the DNA-dependent protein kinase (DNA-PK) complex to the DNA and, when DNA bound, can interact and activate the DNA-PK catalytic subunit (DNA-PKcs). We have carried out a 3' deletion ... Ku protein binds broken DNA ends, triggering a double-strand Abstract. Ku is an ubiquitous nuclear heterodimeric pro If peanut IgE is 0.10 kU/L or greater, then 7 peanut components (Ara h 2, Ara h 1, Ara h 3, Ara h 6, Ara h 8, Ara h 9, and profilin Bet v2) are performed at an additional charge. ... Group 1 Fagales-related protein (h 8) PR-10 Protein (h 8) Non-specific Lipid Transfer Protein (ns-LTP) (h 9) PR-14 Protein (h 9) Bet v2. Profilin. Although Ku protein stabilizes DNA-PKcs binding overall, it The Ku autoantigen is a heterodimer of 70 (p70) and approximately 80 kDa (p80) subunits that is the DNA-binding component of the DNA-dependent protein kinase (DNA-PK) complex involved in DNA ...Abstract. Ku70, Ku80, and DNA-PKcs are subunits of the DNA-dependent protein kinase (DNA-PK), an enzyme implicated in DNA double-stranded break repair and V (D)J recombination. Our Ku70-deficient mice were about 50% the size of control littermates, and their fibroblasts were ionizing radiation sensitive and displayed premature senescence ... Ku is also present in the cytoplasm and at the meKoagulasi protein telur dapat terjadi karena panas, garam, aHendzel and his lab discovered that a poorly characterized protein c KU80; Ku-core domain, Ku80 subfamily; Ku80 is a subunit of the Ku protein, which plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V (D)J recombination. The mechanism underlying the ... pfam08785. Location:558 → 672.Sep 2, 2021 · The effect of MCL1 on DSBs goes beyond its effects on DDR protein foci accumulation. MCL1 binds and inhibits Ku, an essential NHEJ protein that stabilizes the DNA ends to promote ligation 54 (Fig ... Ku, another protein required for NHEJ, also stimulates LX Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit and 83,528 Dalton for the p80 subunit. Ku (P70/P80) is expressed with a -6xHis tag and purified by ... The level of Ku protein was assessed by Western blot with anti-Ku ant[The DNA end-binding activity of Ku may also bAra h 3. • Sensitization to Ara h 1, 2 and 3 is usually The Ku protein usually used for in vitro studies is either an endogenous protein purified from mammalian cells or a protein produced in the baculovirus system [56, 57].Both approaches to Ku production are multi-step and result in relatively low yields and high costs. Purification from E.coli cells is mainly used only for truncated Ku proteins [58, 59].