Pacific blue fluorophore
The LIVE/DEAD Fixable Red (615) Viability kit for 488 and 561 nm excitation was used to differentially stain a mixture of live (left peak) and heat-treated Jurkat cells (right peak). Cells in (A) were not fixed; cells in (B) were fixed in 3.7% formaldehyde following staining. Samples were analyzed by flow cytometry using 488 nm excitation and ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... Brilliant™ Violet 510 (BV510) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 510/50 bandpass filter. BV510 has an excitation peak at 405 nm and an emission peak at 510 nm, and is similar to StarBright Violet 515 (Bio-Rad). BV510 is most often used in flow cytometry due ...
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Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low ...Fluorophore and reagent selection guide for flow cytometry Excitation laser Common emission filters (nm) Attune NxT channel (nm)* Recommended dyes ... Pacific Blue Alexa Fluor 405 LIVE/DEAD Fixable Violet DAPI SYTOX Blue Vybrant DyeCycle Violet FxCycle Violet Annexin V, Pacific Blue Annexin V, eFluor 450 PO-PRO-1Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low ...
See the characteristics of Pacific Blue dye and the range of antibody conjugates, kits, and other products related to this fluorophore. Quantification of Small Molecule-Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore. ...Choose fluorophores with emissions having the least spectral overlap. For example, although Brilliant Violet 421™ and Pacific Blue™ do not have exact the same emission spectra, they did have significant overlap, so you should generally avoid using these together. Fluorophore type. Organic dye. Organic dye. Organic dye (tandem), resonance energy transfer from PE molecule to eFluor 610 dye. Organic dye (tandem), resonance energy transfer from PerCP molecule to eFluor 710 dye. Ex (nm) 475. 546. 496, 565. 488. Em (nm) 535. 585. 607. 710. Application. ICC, IHC. ICC, IHC. FC. FC. Conjugates available : eFluor ...
Pacific Blue: 455: 500–600: Pacific Green: 500 : Horizon V500, Brilliant Violet 510, VioGreen, Brilliant Violet 570: eFluor 506: 510: Pacific Orange: 550: 600–700: Super Bright 600: 600 : Brilliant Violet 605, ... Fluorophore staining index …Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...Cytometer LASER emission Detector range Fluorochrome Names check Accuri C6 Blue 488 FL3 670+ PerCP PI (Viability) 7-AAD PerCP-Cy5.5 PerCP-eFluor710 PE-Cy7 PE-Vio770 PE-Cy5.5 FL2 565-605 PE PI (cell cycle) ... 2 449-471 Pacific Blue CFP Live dead violet Qdot 525 V450 eFluor450 1 415-425 BV421 Blue 488 5 750+ BB790 PE-Cy7 4 652-732 PerCP-Cy5.5 ...…
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Pacific Blue, or systematically 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. Its excitation maximum lies at 401 nm, while its emission maximum is at 452 nm. [2] In contrast to the less acidic 7-hydroxy-3-carboxycoumarin (pKa=7.0), the high acidity of the phenol of Pacific Blue (pKa=3.7) causes its ... Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...
An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant.Fluorescence Emission Fluorochrome Color Ex-Max (nm) Analyzers Sorters Excitation Laser Line (nm)† Em-Max (nm) BD Life Sciences Fluorochrome Specifications * Capable of detecting up to 8 colors simultaneously (488-nm laser available on all configurations; 405-nm and 640-nm lasers available on select configurations). ** Capable of detecting up to 12 colors …
hermes grace We would like to show you a description here but the site won’t allow us. administrative problems in schoolsmeloco kyoran past life Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.* The excitation laser line represents commonly used lasers that excite the fluorochrome. It does not necessarily reflect the lasers available for each particular instrument. † APC and PE-Cy5 may be used together on instruments with cross-beam compensation. ... Pacific Blue ™ Blue 401 405, 407 ... the game today basketball Choose fluorophores with emissions having the least spectral overlap. For example, although Brilliant Violet 421™ and Pacific Blue™ do not have exact the same emission spectra, they did have significant overlap, so you should generally avoid using these together.Pacific Blue® (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm Violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. ku weight loss programcar urban dictionarykansas volleyball rankings Pacific Blue (ex 410/em 455) Pacific Blue is a blue, fluorescent, coumarin based dye, excitable by the 405 nm laser. It emits at 455 nm and is best used for markers that are highly expressed due to its dim nature. This fluorophore is not compatible with StarBright Violet 440 Dye due to similar emission spectra and spillover characteristics.The LIVE/DEAD™ Fixable Blue Stain was selected based on its fluorescent properties to provide a bright signal when excited with a UV laser. The blue-fluorescent reactive dye has an excitation maximum of ∼350 nm, making it ideal for use with an UV laser, and an emission of ∼450 nm. By switching viability staining from a channel off of the ... jazzy select elite manual Pacific Orange Dye Cascade Blue and Other Pyrene Derivatives Cascade Yellow and Other Pyridyloxazole Derivatives Naphthalenes, Including Dansyl Chloride Dapoxyl Dye Bimane Data Table Ordering Information Related Tables Molecular Probes amine-reactive dyes—Table 1.1 Active esters and kits for labeling proteins and nucleic acids—Table 1.2 pharmacy bradleyosrs varrock museum quiz2005 ford escape serpentine belt replacement c Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, ... In this work, a blue fluorophore 5-oxopyrrolidine-3-carboxylic acid (1) has been fully isolated from a citric acid and urea CD synthesis and characterized with NMR, electrospray ionization-mass spectrometry (ESI-MS), and Fourier-transform ...Pacific Blue® (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm Violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. Spectra Alternative Dyes to Pacific Blue Check to add to spectra viewer Pacific Blue V450 ViaFluor 405